Analytical procedure for the determination of tetracyclines in medicated feedingstuffs by liquid chromatography-mass spectrometry

Analytical procedure for the determination of tetracyclines in medicated feedingstuffs by liquid chromatography-mass spectrometry

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Introduction: The article presents a rapid and simple analytical procedure for determination of four tetracyclines (oxytetracycline, tetracycline, chlortetracycline, and doxycycline) in animal medicated feedingstuffs.Material and Methods: Two-gramme samples were extracted by a Na2EDTA-McIlvaine buffer (pH 4)/methanol mixtures (40/60, v/v).The determination was achieved by liquid chromatography using a Zorbax Eclipse XDB C18 analytical column with SPF 40 ADULT mass spectrometer detection (LC-MS).Results: Recoveries of the antibiotics from spiked feed samples ranged from 78.

2% to 113.5%.The LOD and LOQ for tetracyclines in feeds ranged from 2.8 to 4.

2 and from 4.3 to 5.7 mg/kg, respectively.Conclusion: The Jewelry Holders method was successfully validated and proved to be efficient, precise, and useful for quantification of tetracyclines in medicated feedingstuffs.